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KMID : 0380020100250030289
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 3 p.289 ~ p.296
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Antifungal Activity and Biochemical Characterization of Lectin Isolated from Locular Fluid of Cherry Tomato Fruit
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Roh Kwang-Soo
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Abstract
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Lectins are carbohydrate-binding and a cell-agglutinating proteins, and are concerted with a plants defence mechanism. In particular, chitin-binding lectins in locular fluid of cherry tomato fruit seemed to have a role in defending plants against fungi. The antifungal activity using lectin isolated from locular fluid of cherry tomato fruit was measured in the plant pathogen Cladosporium cucumerinum, Monosporascus cannonballus, Fusarium oxysporum, and Rhizoctonia solani. Amoung the four strains, a potent antifungal activity was detected in Cladosporium cucumerinum and Monosporascus cannonballus, not in Fusarium oxysporum, and Rhizoctonia solani. The molecular weight of this lectin isolated as double protein bands by SDS-PAGE was calculated to be 87 kDa and 47 kDa from the relative mobilities compared with those of reference molecular weight markers. The isolated lectin agglutinated human red blood cells (A, B, AB, O) treated with trypsin, and the most activity was found at B. The optimal temperature of isolated lectin was at 30℃. For the thermal stability, lectin was stable at 20-80℃. The optimal pH of this lectin was at 7.2, and showed complete loss below pH 9.0.
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KEYWORD
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Antifungal activity, cherry tomato, Cladosporium cucumerinum, locular fluid lectin, Monosporascus cannonballus
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